Evaluation of haematinic activity of Tapinanthus globiferus (A. Rich.) van Tiegh leaf extract and fractions in phenylhydrazine-induced anaemic rats

Abstract

Anaemia is a leading cause of morbidity and mortality in both males and females of all ages. Anaemia was induced by intraperitoneal (i.p.) administration of phenylhydrazine (60 mg/kg) for a period of 3 days. Anaemic rats were allotted to five (5) groups; group I served as the negative control, group II served as the positive control received Astyfer syrup (0.15 mg/kg p.o) , groups III-V served as the test groups that received Tapinanthus globiferus methanol extract, ethyl acetate and n-hexane fractions (100, 200 and 400 mg/kg, p.o.), respectively. Packed cell volume (PCV), haemoglobin concentration (HB) and red blood cell count (RBC) were analysed as indices of anaemia at 7-day intervals for 21 days. The mean cell volume (MCV) and mean cell haemoglobin concentration (MCHC) were calculated accordingly. Leaf extract and fractions of Tapinanthus globiferus significantly (P<0.001) increased the PCV, the HB concentration and the RBC, which had been originally decreased by phenylhydrazine (P<0.001) administration within one week of treatment. The extract and fractions were compared with rats administered with the positive and vehicle-treated group. The results suggested that Tapinanthus globiferus leaves have haematinic activities.